ALKBH3 Human
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, ALKBH3, ABH3, DEPC1, PCA1, FLJ43614, MGC118790, MGC118792, MGC118793.
Description
ALKBH3 Human Recombinant fused to 39 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 325 amino acids (1-286 a.a.) and having a molecular mass of 37.9kDa.
The ALKBH3 is purified by proprietary chromatographic techniques.
Product Specs
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, ALKBH3, ABH3, DEPC1, PCA1, FLJ43614, MGC118790, MGC118792, MGC118793.
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW.
Product Science Overview
ALKBH3 plays a crucial role in the repair of alkylated DNA and RNA. It specifically targets and repairs lesions such as 1-methyladenine (m1A) and 3-methylcytosine (m3C) through a process known as oxidative demethylation . This repair mechanism is vital for maintaining the integrity of genetic information and preventing mutations that could lead to diseases such as cancer .
The protein exhibits a strong preference for single-stranded DNA and RNA substrates . It can also process alkylated m3C within double-stranded regions by interacting with ASCC3, which promotes DNA unwinding to generate the single-stranded substrate needed for ALKBH3 activity .
Mutations or dysregulation of the ALKBH3 gene have been associated with various diseases, including prostate cancer and familial erythrocytosis . The protein’s role in repairing DNA damage makes it a potential target for cancer therapies, as inhibiting its activity could enhance the effectiveness of alkylating agents used in chemotherapy .
Recombinant ALKBH3 proteins are produced using recombinant DNA technology, which involves inserting the ALKBH3 gene into an expression system to produce the protein in large quantities . These recombinant proteins are used in research to study the protein’s function, mechanism, and potential therapeutic applications .
