ALK/P80 Antibody

ALK/P80 Protein, Mouse Anti Human
Cat. No.
BT12213
Source
Synonyms
ALK/P80 Protein, ALK/P80, p80 protein, anaplastic lymphoma kinase, ALK, nucleophosmin, NPM.
Appearance
Purity
Usage
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Description

Product Specs

Introduction
ALK/p80 is a human protein composed of a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene. This hybrid protein arises from a t(2;5)(p23;q35) translocation, which is observed in approximately one-third of large cell lymphomas.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a solution of phosphate-buffered saline (PBS) at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage of up to 1 month, the antibody should be kept at 4°C. For extended storage, it is recommended to store the antibody at -20°C. To maintain the integrity of the antibody, avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody exhibits stability for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
The ALK/P80 antibody has been rigorously validated for specificity and reactivity across various applications, including ELISA, Western blot, and immunohistochemical analysis. While the antibody has been optimized for performance, it's essential to determine the optimal working concentration for each specific application through titration. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
ALK/P80 Protein, ALK/P80, p80 protein, anaplastic lymphoma kinase, ALK, nucleophosmin, NPM.
Purification Method
ALK/P80 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P5E3AT.
Immunogen
Anti-human ALK/P80 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ALK/P80 amino acids 81-294 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

The ALK/P80 protein, also known as Anaplastic Lymphoma Kinase (ALK), is a receptor tyrosine kinase that plays a crucial role in the development of the nervous system. The mouse anti-human ALK/P80 antibody is a monoclonal antibody used in various research and diagnostic applications to detect the presence of ALK/P80 protein in human tissues.

ALK/P80 Protein

The ALK gene is located on chromosome 2p23 and encodes a receptor tyrosine kinase that is involved in cell growth and differentiation. The ALK protein is normally expressed in the nervous system, but its aberrant expression has been linked to various cancers, including anaplastic large cell lymphoma (ALCL) and non-small cell lung cancer (NSCLC).

NPM-ALK Fusion Protein

In a subset of ALCL cases, a chromosomal translocation t(2;5)(p23;q35) occurs, resulting in the fusion of the ALK gene with the nucleophosmin (NPM) gene. This fusion gene encodes the NPM-ALK fusion protein, also known as p80. The NPM-ALK fusion protein is constitutively active, leading to uncontrolled cell proliferation and survival, which contributes to the development of lymphoma .

Mouse Anti-Human ALK/P80 Antibody

The mouse anti-human ALK/P80 antibody is a monoclonal antibody that specifically recognizes the ALK/P80 protein. It is commonly used in immunohistochemistry (IHC) to detect ALK/P80 expression in formalin-fixed, paraffin-embedded tissue sections. This antibody is valuable in diagnosing ALCL and other ALK-positive malignancies .

Applications
  1. Diagnostic Tool: The mouse anti-human ALK/P80 antibody is used in clinical diagnostics to identify ALK-positive tumors. It helps pathologists determine the presence of ALK rearrangements in tumor samples, which can guide treatment decisions.
  2. Research: In research settings, this antibody is used to study the role of ALK in cancer development and progression. It is also used to investigate the molecular mechanisms underlying ALK-positive malignancies .

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THE BIOTEK
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