AKT3 Human
HEK293 Cells.
RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, AKT3, PKBG.
Sterile Filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
AKT3 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 485 amino acids (1-479a.a) and having a molecular mass of 56.5kDa.
AKT3 is fused to a 6 amino acid His-tag at C-terminus and is purified by proprietary chromatographic techniques.
Product Specs
AKT Serine/Threonine Kinase 3 (Akt3) is a member of the Akt subfamily of serine/threonine protein kinases. Akt3 is one of three closely related serine/threonine-protein kinases (Akt1, Akt2, and Akt3) known as the Akt kinase, which regulate various cellular processes including proliferation, metabolism, cell survival, growth, and angiogenesis. This regulation is mediated through the phosphorylation of serine and/or threonine residues on a range of downstream substrates. Akt3 plays a role in brain development and is essential for the survival of malignant glioma cells.
Recombinant human AKT3, expressed in HEK293 cells, is a single, glycosylated polypeptide chain comprising 485 amino acids (1-479a.a) with a molecular weight of 56.5 kDa.
A 6-amino acid His-tag is fused to the C-terminus of AKT3. The protein is purified using proprietary chromatographic techniques.
Sterile, colorless solution.
The AKT3 solution (0.25 mg/ml) is supplied in PBS (pH 7.4) with 10% glycerol.
For short-term storage (2-4 weeks), store at 4°C.
For long-term storage, freeze at -20°C.
Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage.
Avoid repeated freeze-thaw cycles.
Greater than 90% purity as determined by SDS-PAGE analysis.
RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, AKT3, PKBG.
HEK293 Cells.
MSDVTIVKEG WVQKRGEYIK NWRPRYFLLK TDGSFIGYKE KPQDVDLPYP LNNFSVAKCQ LMKTERPKPN TFIIRCLQWT TVIERTFHVD TPEEREEWTE AIQAVADRLQ RQEEERMNCS PTSQIDNIGE EEMDASTTHH KRKTMNDFDY LKLLGKGTFG KVILVREKAS GKYYAMKILK KEVIIAKDEV AHTLTESRVL KNTRHPFLTS LKYSFQTKDR LCFVMEYVNG GELFFHLSRE RVFSEDRTRF YGAEIVSALD YLHSGKIVYR DLKLENLMLD KDGHIKITDF GLCKEGITDA ATMKTFCGTP EYLAPEVLED NDYGRAVDWW GLGVVMYEMM CGRLPFYNQD HEKLFELILM EDIKFPRTLS SDAKSLLSGL LIKDPNKRLG GGPDDAKEIM RHSFFSGVNW QDVYDKKLVP PFKPQVTSET DTRYFDEEFT AQTITITPPE KYDEDGMDCM DNERRPHFPQ FSYSASGREH HHHHH.
Product Science Overview
The AKT3 gene is located on chromosome 1q43-q44 in humans . The protein encoded by this gene is one of three closely related AKT isoforms, the others being AKT1 and AKT2 . AKT3 is the least studied among the AKT isoforms but is known to be essential for brain development and the viability of malignant glioma cells .
AKT3 is involved in regulating cell signaling in response to insulin and growth factors such as platelet-derived growth factor (PDGF) and insulin-like growth factor 1 (IGF1) . It mediates its effects through serine and/or threonine phosphorylation of a range of downstream substrates . This kinase has been shown to play a role in various biological processes, including:
AKT3 has been implicated in several critical biological processes:
- Brain Development: AKT3 is crucial for brain morphogenesis and the maintenance of the number of cells within a tissue .
- Cancer: It is essential for the viability of malignant glioma cells and may play a role in the regulation of matrix metalloproteinase 13 (MMP13) via interleukin 13 (IL13) .
- Angiogenesis: AKT3 positively regulates endothelial cell proliferation and migration, contributing to angiogenesis .
