AKR1C4 Human, His
Aldo-Keto Reductase Family 1 Member C4 Human Recombinant, His Tag
Cat. No.
BT16609
Source
Escherichia Coli.
Synonyms
Aldo-keto reductase family 1 member C4 (chlordecone reductase 3-alpha hydroxysteroid dehydrogenase type I dihydrodiol dehydrogenase 4), 3-alpha-hydroxysteroid dehydrogenase type I, MGC22581, HAKRA, 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4, CDR, DD4, CHDR, 3-alpha-HSD1, C11.
Appearance
AKR1C4 is supplied as a sterile filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
AKR1C4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-323) and having a molecular mass of 39.2 kDa.
The AKR1C4 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
The AKR1C4 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
AKR1C4, a member of the aldo/keto reductase superfamily with over 40 known enzymes and proteins, catalyzes the reduction of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. This enzyme plays a crucial role in detoxifying the organochlorine pesticide chlordecone by converting it to chlordecone alcohol in the liver.
Description
Recombinant human AKR1C4, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 343 amino acids (1-323) with a molecular weight of 39.2 kDa. A 20 amino acid His-Tag is fused to the N-terminus to facilitate purification via proprietary chromatographic techniques.
Physical Appearance
AKR1C4 is provided as a clear, sterile solution after filtration.
Formulation
AKR1C4 protein is supplied at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8), 0.1M NaCl, 1mM DTT, and 20% Glycerol.
Stability
For short-term storage (2-4 weeks), AKR1C4 should be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of AKR1C4 is determined to be greater than 90% using SDS-PAGE analysis.
Biological Activity
The specific activity of AKR1C4, defined as the amount of enzyme required to catalyze the oxidation of 1.0 pmole of 1-Acenaphthenol per minute in the presence of NADP at pH 8.8 and 25°C, is greater than 700 pmol/min/µg.
Synonyms
Aldo-keto reductase family 1 member C4 (chlordecone reductase 3-alpha hydroxysteroid dehydrogenase type I dihydrodiol dehydrogenase 4), 3-alpha-hydroxysteroid dehydrogenase type I, MGC22581, HAKRA, 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4, CDR, DD4, CHDR, 3-alpha-HSD1, C11.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA
KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY.
KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY.
Product Science Overview
Introduction
Gene and Protein Structure
Biological Functions
AKR1C4 plays a crucial role in the metabolism of steroid hormones and bile acids. It catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in the liver . Additionally, it is involved in the transformation of dihydrotestosterone, a potent natural androgen, into 5-alpha-androstan-3-alpha,17-beta-diol, a compound with much lower activity .
Recombinant Protein
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