AKR1C1 Antibody

Aldo-Keto Reductase Family 1 Member C1, Mouse Anti Human
Cat. No.
BT13423
Source
Synonyms
DDH1, DDH, HAKRC, 20-alpha-HSD, DD1/DD2, HBAB, C9, DD1, H-37, MBAB, MGC8954, 2-ALPHA-HSD, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, Indanol dehydrogenase, Dihydrodiol dehydrogenase 1/2, Chlordecone reductase homolog HAKRC, High-affinity hepatic bile acid-binding protein.
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Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
AKR1C1, an enzyme of the aldo/keto reductase superfamily, converts progesterone to its inactive form, 20-alpha-hydroxy progesterone (20-alpha-OHP). Primarily found in the liver and intestine, it plays a role in bile acid metabolism and myelin formation. While exhibiting low bile-binding affinity, it contributes to regulating intrahepatic bile acid concentrations. AKR1C1's catalytic activity, similar to other enzymes in the 40+ member superfamily, involves converting aldehydes and ketones to their respective alcohols using NADH and/or NADPH as cofactors. This results in overlapping yet distinct substrate specificity within the superfamily.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be stored at 4°C. For extended storage, it is recommended to store at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C. At 4°C, it remains stable for 1 month.
Applications
The AKR1C1 antibody has been rigorously tested and validated for specificity and reactivity using both ELISA and Western blot analysis. While recommended dilutions for Western blot analysis start at 1:1000, optimal working concentrations may vary depending on the specific application and should be determined through titration.
Synonyms
DDH1, DDH, HAKRC, 20-alpha-HSD, DD1/DD2, HBAB, C9, DD1, H-37, MBAB, MGC8954, 2-ALPHA-HSD, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, Indanol dehydrogenase, Dihydrodiol dehydrogenase 1/2, Chlordecone reductase homolog HAKRC, High-affinity hepatic bile acid-binding protein.
Purification Method

AKR1C1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT6D10AT.

Immunogen

Anti-human AKR1C1 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human AKR1C1 amino acids 1-323 purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Introduction

Aldo-Keto Reductase Family 1 Member C1 (AKR1C1) is a protein-coding gene that belongs to the aldo-keto reductase superfamily. This superfamily consists of more than 40 known enzymes and proteins that catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors . The enzymes in this family display overlapping but distinct substrate specificity .

Gene and Protein Information

The AKR1C1 gene is located on chromosome 10p15-p14 and shares high sequence identity with three other gene members clustered in the same region . The protein encoded by this gene is involved in the metabolism of steroid hormones, including the conversion of progesterone to its inactive form, 20-alpha-hydroxy-progesterone . AKR1C1 is also known by several other names, including Dihydrodiol Dehydrogenase 1, 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase, and High-Affinity Hepatic Bile Acid-Binding Protein .

Function and Pathways

AKR1C1 plays a crucial role in various biological processes, including the synthesis of bile acids and bile salts, and visual phototransduction . The enzyme’s oxidoreductase activity is essential for its function in these pathways . Additionally, AKR1C1 is involved in the regulation of osteogenic differentiation of human adipose-derived mesenchymal stromal/stem cells (hASCs) by targeting the progesterone receptor . This regulation is significant for bone tissue engineering and the repair of bone defects .

Clinical Significance

Mutations or dysregulation of the AKR1C1 gene have been associated with several diseases, including developmental dysplasia of the hip and lipedema . The enzyme’s role in steroid hormone metabolism also links it to various tumors, making it a potential target for cancer research and therapy .

Mouse Anti Human AKR1C1 Antibody

The mouse anti-human AKR1C1 antibody is a monoclonal antibody used in research to study the expression and function of the AKR1C1 protein in human tissues. This antibody is commonly used in immunohistochemistry (IHC) to detect AKR1C1 in formalin-fixed, paraffin-embedded human hepatocellular carcinoma samples . The antibody’s specificity and binding affinity make it a valuable tool for investigating the role of AKR1C1 in various biological and pathological processes.

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