AIF1L Human

Allograft Inflammatory Factor 1 Like Human Recombinant
Cat. No.
BT8939
Source
Escherichia Coli.
Synonyms
Allograft Inflammatory Factor 1-like, IBA2, FLJ12783, Ionized calcium-binding adapter molecule 2, C9orf58, chromosome 9 open reading frame 58, MGC29466.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

AIF1L produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (1-150a.a.) and having a molecular mass of 19.2kDa.
AIF1L is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Description
The AIF1L protein, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 170 amino acids (specifically, amino acids 1 through 150). Its molecular mass is 19.2kDa. The AIF1L protein has a 20 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized through filtration.
Formulation
The AIF1L protein is provided in a solution at a concentration of 1mg/ml. The formulation buffer is 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM NaCl, and 20% glycerol.
Stability
For optimal storage, keep the entire vial at 4°C if it will be used within 2-4 weeks. For longer storage periods, freeze the product at -20°C. To ensure stability during long-term storage, it is advisable to add a carrier protein such as HSA or BSA at a concentration of 0.1%. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the AIF1L protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Allograft Inflammatory Factor 1-like, IBA2, FLJ12783, Ionized calcium-binding adapter molecule 2, C9orf58, chromosome 9 open reading frame 58, MGC29466.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGVS DTISYRDFVN MMLGKRSAVL KLVMMFEGKA NESSPKPVGP PPERDIASLP.

Product Science Overview

Introduction

Allograft Inflammatory Factor 1 Like (AIF1L) is a protein encoded by the AIF1L gene in humans. This protein is part of the allograft inflammatory factor family and is known for its role in actin binding and bundling. AIF1L is an actin-binding protein that promotes actin bundling and is involved in various cellular processes, including cytoskeletal organization and cell motility .

Gene and Protein Structure

The AIF1L gene is located on chromosome 9 in humans, specifically at the 9q34.12-q34.13 region . The gene spans approximately 26,676 base pairs and encodes a protein that consists of 147 amino acids. The protein has a molecular weight of approximately 17 kDa .

Expression and Localization

AIF1L is expressed in various tissues, with a biased expression in the kidney, spleen, and several other tissues . The protein is primarily localized in the cytoplasm and is associated with the actin cytoskeleton. It is also found in the plasma membrane, cell projections, and extracellular exosomes .

Function

AIF1L is an actin-binding protein that promotes actin bundling, which is essential for maintaining the structural integrity of the cytoskeleton. Unlike other members of the allograft inflammatory factor family, AIF1L does not bind calcium and does not depend on calcium for its function . The protein plays a crucial role in various cellular processes, including actin filament bundle assembly and ruffle assembly .

Biological Significance

AIF1L is involved in several biological processes, including cytoskeletal organization, cell motility, and intracellular signaling. The protein’s ability to bind and bundle actin filaments is critical for maintaining the structural integrity of the cytoskeleton and facilitating cellular movements . Additionally, AIF1L is implicated in various physiological and pathological conditions, including inflammation and immune responses .

Clinical Relevance

Research has shown that AIF1L is associated with several inflammatory diseases and conditions. The protein’s role in actin bundling and cytoskeletal organization makes it a potential target for therapeutic interventions in diseases characterized by cytoskeletal abnormalities and inflammation . Further studies are needed to fully understand the clinical implications of AIF1L and its potential as a therapeutic target.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.