AIDA Human

Axin Interactor Dorsalization Associated Human Recombinant

Cat. No.

BT23170

Source

E.coli.

Synonyms

C1orf80, RP11-378J18.7, Axin interactor, dorsalization-associated protein, Axin interaction partner and dorsalization antagonist, AIDA.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

AIDA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 329 amino acids (1-306 a.a) and having a molecular mass of 37.4kDa. AIDA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Axin interactor, dorsalization-associated (AIDA), also known as Ankyrin repeat domain-containing protein 11, functions as a ventralizing factor during embryonic development. It inhibits axin-mediated JNK activation through binding to axin and disrupting its homodimerization. This action antagonizes a Wnt/beta-catenin-independent dorsalization pathway that is activated by AXIN/JNK signaling.

Description

Recombinant human AIDA protein was expressed in E. coli and purified to a single, non-glycosylated polypeptide chain. This protein contains 329 amino acids, consisting of AIDA residues 1-306 and an N-terminal 23 amino acid His-tag. The molecular weight of the AIDA protein is 37.4 kDa. Purification was achieved using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless and sterile filtered solution.

Formulation

The AIDA protein is supplied in a solution at a concentration of 0.25 mg/ml. The formulation buffer contains 20 mM Tris-HCl (pH 8.0), 10% glycerol, 0.2 M NaCl, and 1 mM DTT.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. It's important to avoid repeated freeze-thaw cycles to maintain protein integrity.

Purity

The purity of the AIDA protein is greater than 90% as determined by SDS-PAGE analysis.

Synonyms

C1orf80, RP11-378J18.7, Axin interactor, dorsalization-associated protein, Axin interaction partner and dorsalization antagonist, AIDA.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSEVTRS LLQRWGASFR RGADFDSWGQ LVEAIDEYQI LARHLQKEAQ AQHNNSEFTE EQKKTIGKIA TCLELRSAAL QSTQSQEEFK LEDLKKLEPI LKNILTYNKE FPFDVQPVPL RRILAPGEEE NLEFEEDEEE GGAGAGSPDS FPARVPGTLL PRLPSEPGMT LLTIRIEKIG LKDAGQCIDP YITVSVKDLN GIDLTPVQDT PVASRKEDTY VHFNVDIELQ KHVEKLTKGA AIFFEFKHYK PKKRFTSTKC FAFMEMDEIK PGPIVIELYK KPTDFKRKKL QLLTKKPLYL HLHQTLHKE.

Product Science Overview

Gene and Protein Information

The AIDA gene is located on chromosome 1 and is a protein-coding gene. The protein encoded by this gene is involved in various cellular processes, including the regulation of the c-Jun N-terminal kinase (JNK) pathway. AIDA acts as a ventralizing factor during embryogenesis by inhibiting axin-mediated JNK activation . This inhibition occurs through the binding of AIDA to axin, which disrupts axin homodimerization. Consequently, AIDA antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.

Functional Role

AIDA’s primary function is to regulate the JNK pathway, which is essential for various cellular processes, including cell proliferation, differentiation, and apoptosis . By inhibiting axin-mediated JNK activation, AIDA plays a critical role in maintaining the balance between ventral and dorsal cell fates during embryonic development . This regulation is vital for proper embryogenesis and the formation of various tissues and organs.

Expression and Localization

AIDA is predicted to be located in the cytoplasm and is active in the membrane. It is expressed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle. The protein’s expression profile suggests its involvement in multiple physiological processes, such as immune response, protein ubiquitination, and spermatid development.

Mechanism of Action

The unique C2 domain of AIDA is responsible for its interaction with axin. This interaction negatively regulates the axin-mediated JNK pathway, thereby modulating cellular signaling and embryonic development. The disruption of axin homodimerization by AIDA is a key mechanism through which it exerts its ventralizing effects.

Clinical Relevance

While the specific clinical implications of AIDA are still under investigation, its role in regulating the JNK pathway and embryogenesis suggests potential involvement in developmental disorders and diseases related to cellular signaling dysregulation . Further research is needed to fully understand the clinical significance of AIDA and its potential as a therapeutic target.

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AIDA Human

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