AFAP1 Human

Actin Filament Associated Protein 1 Human Recombinant
Cat. No.
BT12247
Source
E.coli.
Synonyms
Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP, Actin Filament Associated Protein 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AFAP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 360 amino acids (250-588) and having a molecular mass of 39.2 kDa.
AFAP1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Actin Filament Associated Protein 1 (AFAP1) is a protein that interacts with Src, a type of protein involved in cell signaling. AFAP1 may play a role in maintaining the structure of actin filaments, which are important for cell shape and movement. AFAP1 is also thought to act as an adaptor protein, linking Src family members to actin filaments. Additionally, AFAP1 is believed to be involved in the development and progression of prostate cancer by influencing cell adhesion and migration.
Description
AFAP1 Human Recombinant is a laboratory-produced version of the human AFAP1 protein. It is manufactured using E. coli bacteria and consists of a single chain of 360 amino acids (specifically amino acids 250-588 of the full protein). This recombinant protein has a molecular weight of 39.2 kDa. For purification and detection purposes, a 21 amino acid His-tag is attached to the N-terminus of the protein. The purification process involves specialized chromatographic techniques.
Physical Appearance
The product is a clear and colorless solution that has been sterilized through filtration.
Formulation
The AFAP1 protein is provided in a solution with a concentration of 0.5 mg/ml. The solution contains 20mM Tris-HCl buffer at a pH of 8.0, 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), keep the solution at 4°C. For longer storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. It's crucial to avoid repeatedly freezing and thawing the solution.
Purity
The purity of the AFAP1 protein is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP, Actin Filament Associated Protein 1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGCSGPVDSE CPPPPSSPVH KAELEKKLSS ERPSSDGEGV VENGITTCNG KEQVKRKKSS KSEAKGTVSK VTGKKITKII SLGKKKPSTD EQTSSAEEDV PTCGYLNVLS NSRWRERWCR VKDNKLIFHK DRTDLKTHIV SIPLRGCEVI PGLDCKHPLT FRLLRNGQEV AVLEASSSED MGRWIGILLA ETGSSTDPEA LHYDYIDVEM SASVIQTAKQ TFCFMNRRVI SANPYLGGTS NGYAHPSGTA LHYDDVPCIN GSLRGKKPPV ASNGVTGKGK TLSSQPKKAD PAAVVKRTGS NAAQYKYGKN RVEADAKRLQ TKEEELLKRK EALRNRLAQL.

Product Science Overview

Gene and Protein Structure

The AFAP1 gene is located on chromosome 4 and is a protein-coding gene. It has multiple transcript variants encoding different isoforms . The protein itself is known to cross-link actin filaments into both network and bundle structures, which is essential for maintaining the cytoskeleton’s integrity .

Function and Mechanism

AFAP1 is a Src binding partner, which means it interacts with Src family kinases. These interactions are vital for cellular signaling pathways that regulate various cellular processes, including cell adhesion, migration, and proliferation . AFAP1 can modulate changes in actin filament integrity in response to cellular signals, thereby playing a role in the formation of cellular structures like lamellipodia .

Clinical Significance

AFAP1 has been implicated in the development and progression of certain cancers, such as prostate adenocarcinoma. It regulates cell-matrix adhesions and migration in cancer cells, making it a potential target for cancer therapy . Additionally, diseases associated with AFAP1 include Multiple Mitochondrial Dysfunctions Syndrome 1 .

Research and Applications

Recombinant human AFAP1 is used in various research applications to study its role in actin filament modulation and its interactions with other proteins. Understanding AFAP1’s function and mechanisms can provide insights into cellular processes and potential therapeutic targets for diseases involving cytoskeletal abnormalities .

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