AES Human

Amino-Terminal Enhancer of Split Human Recombinant
Cat. No.
BT22944
Source
Escherichia Coli.
Synonyms
Amino-terminal enhancer of split, Amino enhancer of split, Gp130-associated protein, GAM, Protein ESP1, Protein GRG, AES, GRG, ESP1, GRG5, TLE5, AES-1, AES-2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AES Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (1-197 a.a.) and having a molecular mass of 24.1kDa. The AES is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Amino-terminal enhancer of split (AES) is a protein belonging to the groucho/TLE family. It can function as a homooligomer or form heterooligomers with other family members to suppress the expression of genes within the family. Additionally, AES can repress gene expression regulated by NFkB and is thought to play a crucial role in initiating and maintaining cell differentiation. AES shares sequence similarity with the amino terminus of the Drosophila enhancer of split groucho protein, which is involved in embryonic neurogenesis. AES protein expression is primarily observed in the fetal brain, liver, lung, heart, and kidney, as well as in adult muscle tissue.
Description
Recombinant human AES protein is fused with a 20 amino acid His tag at its N-terminus and produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 217 amino acids (residues 1-197) with a molecular weight of 24.1 kDa. The AES protein undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The AES solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure long-term stability during frozen storage, consider adding a carrier protein like HSA or BSA (0.1%). It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of the AES protein is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Amino-terminal enhancer of split, Amino enhancer of split, Gp130-associated protein, GAM, Protein ESP1, Protein GRG, AES, GRG, ESP1, GRG5, TLE5, AES-1, AES-2.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQAHLSKEDK NGHDGDTHQE DDGEKSD.

Product Science Overview

Introduction

The Amino-Terminal Enhancer of Split (AES), also known as TLE family member 5 (TLE5), is a protein that plays a crucial role in transcriptional repression. It is part of the Groucho/TLE family of proteins, which are known for their involvement in various developmental processes and cellular functions.

Structure and Expression

AES is a human full-length protein, consisting of 197 amino acids . It is expressed in Escherichia coli for recombinant purposes and has a purity of over 95%, making it suitable for various applications such as SDS-PAGE and mass spectrometry . The protein is tagged with a His tag at the N-terminus to facilitate purification and detection .

Biological Functions

AES functions as a transcriptional corepressor, meaning it can inhibit the expression of certain genes by binding to transcription factors and preventing them from activating target genes . It acts as a dominant repressor towards other family members and is known to inhibit NF-kappa-B-regulated gene expression . This inhibition is crucial for maintaining the differentiated state of cells and is essential for the transcriptional repressor activity of SIX3 during retina and lens development .

Mechanism of Action

AES can function as a homooligomer or as a heterooligomer with other Groucho/TLE family members . By forming these complexes, AES can effectively repress the expression of target genes. The protein’s ability to interact with other family members allows it to exert a dominant-negative effect, thereby modulating the activity of other transcriptional repressors .

Clinical Relevance

The role of AES in transcriptional repression and its involvement in developmental processes make it a protein of interest in various research fields. Its ability to inhibit NF-kappa-B-regulated gene expression suggests potential implications in inflammatory responses and cancer . Additionally, its essential role in retina and lens development highlights its importance in developmental biology .

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