ADPRHL2 Human

ADP-Ribosylhydrolase Like 2 Human Recombinant
Cat. No.
BT26803
Source
E.coli.
Synonyms
Poly(ADP-ribose) glycohydrolase ARH3, ADP-ribosylhydrolase 3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, ADPRHL2, ARH3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ADPRHL2 Human Recombinant produced in E. coli is a single polypeptide chain containing 387 amino acids (1-363) and having a molecular mass of 41.5kDa.
ADPRHL2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ADP-ribosylhydrolase like 2 (ADPRHL2) is a member of the ADP-ribosylglycohydrolase family that catalyzes the removal of ADP-ribose from proteins. This enzyme is found in various tissues and plays a critical role in reversing ADP-ribosylation, a modification involved in cellular processes such as DNA repair and cell signaling. ADPRHL2 utilizes magnesium as a cofactor and is primarily located in the mitochondria, as well as the nucleus and cytoplasm of cells.
Description
Recombinant human ADPRHL2, expressed in E. coli, is a single polypeptide chain with a molecular weight of 41.5 kDa. The protein consists of 387 amino acids, with a truncated sequence spanning residues 1 to 363. A 24 amino acid His-tag is fused to the N-terminus to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ADPRHL2 protein is supplied at a concentration of 0.5 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of the ADPRHL2 protein is greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
Poly(ADP-ribose) glycohydrolase ARH3, ADP-ribosylhydrolase 3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, ADPRHL2, ARH3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAAAM AAAAGGGAGA ARSLSRFRGC LAGALLGDCV GSFYEAHDTV DLTSVLRHVQ SLEPDPGTPG SERTEALYYT DDTAMARALV QSLLAKEAFD EVDMAHRFAQ EYKKDPDRGY GAGVVTVFKK LLNPKCRDVF EPARAQFNGK GSYGNGGAMR VAGISLAYSS VQDVQKFARL SAQLTHASSL GYNGAILQAL AVHLALQGES SSEHFLKQLL GHMEDLEGDA QSVLDARELG MEERPYSSRL KKIGELLDQA SVTREEVVSE LGNGIAAFES VPTAIYCFLR CMEPDPEIPS AFNSLQRTLI YSISLGGDTD TIATMAGAIA GAYYGMDQVP ESWQQSCEGY EETDILAQSL HRVFQKS.

Product Science Overview

Structure and Expression

ADPRHL2 is a recombinant protein that is typically produced in E. coli. It consists of a single polypeptide chain containing 387 amino acids, with a molecular mass of approximately 41.5 kDa . The protein is often tagged with a His-tag at the N-terminus to facilitate purification .

Function and Mechanism

ADPRHL2 is ubiquitously expressed in human tissues and utilizes magnesium as a cofactor to catalyze the hydrolysis of poly (ADP-ribose) (PAR), which is synthesized following DNA damage . This hydrolysis is essential for the reversal of ADP-ribosylation, a post-translational modification involved in various cellular processes including DNA repair, genomic stability, and regulation of protein function .

Biological Significance

The enzyme’s activity is particularly important in the context of DNA damage response. By removing ADP-ribose groups from proteins, ADPRHL2 helps to maintain normal neuronal cell function and overall cellular homeostasis . This function is critical for preventing the accumulation of DNA damage and ensuring proper cellular repair mechanisms.

Research and Applications

Recombinant ADPRHL2 is widely used in research to study the mechanisms of ADP-ribosylation and its reversal. It is also employed in various biochemical assays to understand the role of ADP-ribosylation in different cellular contexts. The availability of recombinant ADPRHL2 allows for detailed studies on its structure, function, and interactions with other cellular components .

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