Complement Factor D is a highly specific protease, and its only known substrate is Factor B in complex with C3. The enzyme catalyzes the initial proteolytic step in the alternative pathway, leading to the activation of the complement system. This activation results in a cascade of events that enhance the ability of antibodies and phagocytic cells to clear pathogens from an organism.
Complement Factor D is expressed at high levels in adipose tissue, which is why it is also referred to as Adipsin. It is secreted by adipocytes into the bloodstream. The protein is a component of the alternative complement pathway, which is best known for its role in humoral suppression of infectious agents.
Recombinant Human Complement Factor D is produced using various expression systems, including mouse myeloma cell lines (NS0-derived) and HEK293 cells. The recombinant protein is typically purified to high levels of purity, often exceeding 95% as determined by SDS-PAGE and other analytical methods. The recombinant form is used in various research applications to study the complement system and its role in immune responses.
Recombinant Complement Factor D is used in research to understand its role in the immune system and its potential therapeutic applications. It is also used in assays to measure its activity and to study its interactions with other components of the complement system.
Recombinant Complement Factor D is usually supplied as a lyophilized powder or a filtered solution. It is stable for several months when stored at -20°C to -80°C under sterile conditions. It is important to avoid repeated freeze-thaw cycles to maintain its activity.