ADI1 Human
Acireductone Dioxygenase 1 Human Recombinant
Cat. No.
BT19789
Source
Escherichia Coli.
Synonyms
APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL, 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase, Fe-ARD, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Submergence-induced protein-like factor.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ADI1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 216 amino acids (1-179 a.a.) and having a molecular mass of 25.6kDa. ADI1 is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Acireductone dioxygenase 1 (ADI1), a member of the acireductone dioxygenase enzyme family, plays a crucial role in methionine salvage. This metal-binding enzyme exhibits diverse functionalities based on its subcellular location, including the regulation of mRNA processing within the nucleus. Notably, ADI1 has been found to suppress MMP14-mediated cell migration. Interestingly, related pseudogenes have been identified on chromosomes 8 and 20.
Description
Produced in E. coli, our ADI1 protein is a non-glycosylated polypeptide chain with a molecular weight of 25.6 kDa. It consists of 216 amino acids, with residues 1 to 179 representing the ADI1 sequence. The protein is expressed with an N-terminal His-tag (37 amino acids) to facilitate purification using proprietary chromatographic methods.
Physical Appearance
The product is a clear, sterile-filtered solution devoid of any color.
Formulation
ADI1 is supplied as a 0.5 mg/ml solution in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of our ADI1 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL, 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase, Fe-ARD, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Submergence-induced protein-like factor.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA.
Product Science Overview
Structure and Production
Function and Mechanism
ADI1 catalyzes two different reactions depending on the metal ion present in its active site . When iron (Fe) is bound, ADI1 produces formate and 2-keto-4-methylthiobutyrate (KMTB), which is a precursor in the methionine recycle pathway . In contrast, when nickel (Ni) is bound, ADI1 produces methylthiopropionate, carbon monoxide, and formate, which do not participate in the methionine recycle pathway .
Localization and Regulation
Genetic Information
Applications and Stability
Recombinant ADI1 is used in various research applications, particularly in studies related to methionine metabolism and enzyme kinetics . For optimal stability, ADI1 should be stored at 4°C if used within 2-4 weeks, or frozen at -20°C for longer periods . It is recommended to avoid multiple freeze-thaw cycles to maintain its activity .
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