ADFP Human

Adipose Differentiation-Related Protein Human Recombinant
Cat. No.
BT22789
Source
Escherichia Coli.
Synonyms
Adipophilin, Adipose differentiation-related protein, ADRP, ADFP, MGC10598.
Appearance
Purity
Greater than 95% as determined by SDS PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ADFP Human Recombinant produced in E.Coli is a signle, non-glycosylated, Polypeptide chain containing 444 amino acids and having a molecular mass of 49 kDa. The protein contains an extra 8 amino acid His tag at N-terminus. The ADFP amino acid sequence is identical to UniProtKB/Swiss-Prot entry Q99541 amino acids 4–437.
The ADFP is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Adipose differentiation-related protein (ADFP) is associated with the globule surface membrane material and is a major component of the globule surface. An increase in ADFP mRNA levels is an early indicator of adipocyte differentiation. Mycobacterium leprae regulates ADFP expression to promote lipid accumulation within infected macrophages for intracellular survival. ADFP is found in lipid droplets of hepatic stellate cells that store vitamin A, as well as in lipid droplets of steatotic hepatocytes. ADFP expression plays a role in clear cell renal carcinoma differentiation. ADFP is a component of the lipid droplets in THP-1 cells.
Description
Recombinant human ADFP, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 444 amino acids (including an 8 amino acid His tag at the N-terminus) with a molecular weight of 49 kDa. The amino acid sequence of this recombinant ADFP is identical to amino acids 4-437 of the UniProtKB/Swiss-Prot entry Q99541. This product is purified using proprietary chromatographic techniques.
Formulation
Lyophilized from a 0.5mg/ml solution containing 20mM Tris pH 7.5, and 20mM NaCl.
Solubility
To prepare a working stock solution of approximately 0.5mg/mL, add deionized water to the lyophilized pellet and allow it to dissolve completely. This product is not sterile. Please filter the reconstituted protein using an appropriate sterile filter before using it in cell culture.
Stability
Store lyophilized protein at -20°C. After reconstitution, aliquot the product to avoid repeated freeze/thaw cycles. Reconstituted protein can be stored at 4°C for a limited period; no changes have been observed after storage at 4°C for two weeks.
Purity
Greater than 95% purity as determined by SDS-PAGE.
Synonyms
Adipophilin, Adipose differentiation-related protein, ADRP, ADFP, MGC10598.
Source
Escherichia Coli.
Amino Acid Sequence

MKHHHHHHAS VAVDPQPSVV TRVVNLPLVS STYDLMSSAY LSTKDQYPYL KSVCEMAENG VKTITSVAMT SALPIIQKLE PQIAVANTYA CKGLDRIEER LPILNQPSTQ IVANAKGAVT GAKDAVTTTV TGAKDSVAST ITGVMDKTKG AVTGSVEKTK SVVSGSINTV LGSRMMQLVS SGVENALTKS ELLVEQYLPL TEEELEKEAK KVEGFDLVQK PSYYVRLGSL STKLHSRAYQ QALSRVKEAK QKSQQTISQL HSTVHLIEFA RKNVYSANQK IQDAQDKLYL SWVEWKRSIG YDDTDESHCA EHIESRTLAI ARNLTQQLQT TCHTLLSNIQ GVPQNIQDQA KHMGVMAGDI YSVFRNAASF KEVSDSLLTS SKGQLQKMKE SLDDVMDYLV NNTPLNWLVG PFYPQLTESQ NAQDQGAEMD KSSQETQRSEHKTH.

Product Science Overview

Structure and Expression

ADRP is a non-glycosylated polypeptide chain consisting of 444 amino acids, with a molecular mass of approximately 49 kDa . The protein contains an extra 8 amino acid His tag at the N-terminus when produced recombinantly in E. coli . The amino acid sequence of ADRP is identical to the UniProtKB/Swiss-Prot entry Q99541 amino acids 4–437 .

Biological Functions

ADRP is primarily associated with lipid droplets in various cell types, including adipocytes, hepatocytes, and macrophages. It plays a significant role in the formation and stabilization of lipid droplets, which are essential for lipid storage and metabolism. The expression of ADRP is upregulated during adipocyte differentiation, making it a marker for this process .

In addition to its role in lipid storage, ADRP is involved in several physiological and pathological processes:

  • Lipid Metabolism: ADRP facilitates the accumulation of lipids within cells, contributing to energy storage and lipid homeostasis.
  • Cellular Differentiation: ADRP expression is one of the initial indicators of adipocyte differentiation. It is also expressed in lipid droplets of vitamin A-storing hepatic stellate cells and steatotic hepatocytes .
  • Disease Association: ADRP expression has been linked to various diseases, including clear cell renal carcinoma and conditions involving lipid metabolism disorders .
Regulatory Mechanisms

The expression of ADRP is regulated by several factors, including hormonal signals and transcription factors. For instance, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα) are key transcription factors that upregulate ADRP expression during adipogenesis .

Recombinant Production

Human recombinant ADRP is produced in E. coli and purified using proprietary chromatographic techniques. The recombinant protein is typically lyophilized from a solution containing 20mM Tris pH-7.5 and 20mM NaCl . It is available in various quantities for research purposes and is used to study lipid metabolism, adipocyte differentiation, and related biological processes.

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