ACTR3 Human

ARP3 Actin-Related Protein 3 Human Recombinant
Cat. No.
BT22691
Source
Escherichia Coli.
Synonyms
ARP3 Actin-Related Protein 3 Homolog (Yeast), ARP3, Actin-Like Protein 3, ARP3 (Actin-Related Protein 3, Yeast) Homolog, Actin-Related Protein 3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACTR3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441 amino acids (1-418 a.a) and having a molecular mass of 49.8kDa.
ACTR3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ARP3 Actin-Related Protein 3, also known as ACTR3, is a member of the actin protein family. While its precise function remains to be fully elucidated, ACTR3 is recognized as a key component of the ARP2/3 complex. This complex, situated at the cell surface, plays a critical role in cell morphology and movement by facilitating the assembly and extension of actin filaments in lamellipodia. Currently, three transcript variants encoding two distinct isoforms of ACTR3 have been identified.
Description
Recombinant human ACTR3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 441 amino acids (residues 1-418). It has a molecular weight of 49.8 kDa. For purification and detection purposes, the ACTR3 protein is tagged with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ACTR3 protein is supplied as a 0.25 mg/ml solution in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 85.0% as assessed by SDS-PAGE analysis.
Synonyms
ARP3 Actin-Related Protein 3 Homolog (Yeast), ARP3, Actin-Like Protein 3, ARP3 (Actin-Related Protein 3, Yeast) Homolog, Actin-Related Protein 3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGRLPA CVVDCGTGYT KLGYAGNTEP QFIIPSCIAI KESAKVGDQA QRRVMKGVDD LDFFIGDEAI EKPTYATKWP IRHGIVEDWD LMERFMEQVI FKYLRAEPED HYFLLTEPPL NTPENREYTA EIMFESFNVP GLYIAVQAVL ALAASWTSRQ VGERTLTGTV IDSGDGVTHV IPVAEGYVIG SCIKHIPIAG RDITYFIQQL LRDREVGIPP EQSLETAKAV KERYSYVCPD LVKEFNKYDT DGSKWIKQYT GINAISKKEF SIDVGYERFL GPEIFFHPEF ANPDFTQPIS EVVDEVIQNC PIDVRRPLYK NIVLSGGSTM FRDFGRRLQR DLKRTVDARL KLSEELSGGR LKPKPIDVQV ITHHMQRYAV WFGGSMLAST PEFYQVCHTK KDYEEIGPSI CRHNPVFGVM S.

Product Science Overview

Introduction

ARP3 (Actin-Related Protein 3) is a crucial component of the ARP2/3 complex, a multiprotein assembly that plays a significant role in the regulation of the actin cytoskeleton. This complex is essential for various cellular processes, including cell shape, motility, and intracellular transport.

Gene and Protein Structure

The gene encoding ARP3 is known as ACTR3. It is a protein-coding gene that produces multiple transcript variants encoding different isoforms . The ARP3 protein is highly conserved across species, indicating its fundamental role in cellular functions.

Function and Mechanism

ARP3, as part of the ARP2/3 complex, is involved in the nucleation of new actin filaments. This process is critical for the formation of branched actin networks in the cytoplasm, which provide the mechanical force necessary for cell motility . The ARP2/3 complex is activated by nucleation-promoting factors (NPFs), which stimulate the complex to initiate actin polymerization .

In addition to its role in the cytoplasm, the ARP2/3 complex also promotes actin polymerization in the nucleus. This nuclear actin polymerization is involved in regulating gene transcription and repairing damaged DNA .

Cellular Localization

The ARP2/3 complex, including ARP3, is primarily located at the cell surface. It is essential for the formation of lamellipodia, which are sheet-like protrusions at the leading edge of migrating cells . These structures are crucial for cell movement and are involved in various physiological processes, such as wound healing and immune responses.

Clinical Significance

Mutations or dysregulation of the ACTR3 gene can lead to various diseases. For example, it has been associated with Wiskott-Aldrich Syndrome and Leber Hereditary Optic Neuropathy with Demyelinating Disease of the Central Nervous System . Understanding the function and regulation of ARP3 is therefore important for developing therapeutic strategies for these conditions.

Recombinant ARP3

Recombinant ARP3 (Human) is produced using recombinant DNA technology, which allows for the expression of the human ARP3 protein in various host systems. This recombinant protein is used in research to study the function of the ARP2/3 complex and its role in actin dynamics. It is also used in drug development and screening assays to identify potential therapeutic targets.

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