ACOT7 Antibody

Acyl-Coenzyme A Thioesterase 7, Mouse Anti Human
Cat. No.
BT12484
Source
Synonyms
Cytosolic acyl coenzyme A thioester hydrolase, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, BACH, CTE-IIa, CTE-II, Long chain acyl-CoA thioester hydrolase, ACOT7, ACT, ACH1, LACH, LACH1, hBACH, RP1-120G22.10.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Acyl-CoA Thioesterase 7 (ACOT7) is a member of the acyl coenzyme family responsible for hydrolyzing the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Research suggests a potential connection between reduced ACOT7 protein expression and the occurrence of mesial temporal lobe epilepsy.
Physical Appearance
Clear, colorless solution that has undergone sterile filtration.
Formulation
The solution contains a concentration of 1mg/ml of ACOT7 antibody dissolved in a buffer solution consisting of PBS at a pH of 7.4, 10% Glycerol, and 0.01% Sodium Azide.
Storage Procedures
For short-term storage of up to one month, maintain the solution at a temperature of 4°C. For extended storage, it is recommended to keep the solution at -20°C. To maintain product integrity, it is crucial to avoid repeated cycles of freezing and thawing.
Stability / Shelf Life
The product has a shelf life of 12 months when stored at -20°C and a shelf life of 1 month when stored at 4°C.
Applications
This antibody has undergone rigorous testing using ELISA and Western blot analysis to ensure its specificity and reactivity. However, it's essential to optimize the reagent titration for each specific application to achieve the best results. For Western blot analysis, a dilution range of 1:500 to 1:2000 is recommended, with a starting dilution of 1:1000 suggested.
Synonyms
Cytosolic acyl coenzyme A thioester hydrolase, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, BACH, CTE-IIa, CTE-II, Long chain acyl-CoA thioester hydrolase, ACOT7, ACT, ACH1, LACH, LACH1, hBACH, RP1-120G22.10.
Purification Method
ACOT7 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1D5A.
Immunogen
Anti-human ACOT7 mAb, clone PAT1D5A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACOT7 protein 1-370 amino acids  purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and Kappa light chain.

Product Science Overview

Mouse Anti-Human Antibodies

Mouse anti-Human antibodies are secondary antibodies that are affinity-purified and have well-characterized specificity for human immunoglobulins . These antibodies are useful in the detection, sorting, or purification of their specified target. They offer increased versatility, enabling users to use many detection systems such as HRP, AP, and fluorescence . Secondary antibodies can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody .

Applications

Mouse anti-Human antibodies are commonly used in various applications, including:

  • Western Blot (WB)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Immunocytochemistry/Immunofluorescence (ICC/IF)
  • Flow Cytometry (Flow)
  • Immunoprecipitation (IP)

Specificity and Cross-Reactivity

These antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified to generate highly specific reagents . They are designed to recognize human IgG and have been demonstrated not to recognize IgG from non-human primate species, nor mouse or rat immunoglobulin .

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Related Products

ACOT7 Human
Acyl-CoA Thioesterase 7 Human Recombinant
Cat. No.
BT18089
Source
Escherichia Coli.
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