ACO1 Human

Aconitase-1 Human Recombinant
Cat. No.
BT22018
Source
Escherichia Coli.
Synonyms
Onitase 1 Soluble, IRP1, IREB1, IREBP, Citrate hydro-lyase, Iron regulatory protein 1, Ferritin repressor protein, Iron-responsive element-binding protein 1, ACONS, Aconitate Hydratase, EC 4.2.1.3, Aconitase.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACO1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 912 amino acids (1-889a.a.) and having a molecular mass of 100.8kDa.
ACO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ACO1, involved in iron sensing, catalyzes the stereo-specific isomerization of citrate to isocitrate through cis-aconitate in the tricarboxylic acid cycle. This process is non-redox-active.
Description
Produced in E. coli, ACO1 is a single, non-glycosylated polypeptide chain consisting of 912 amino acids (1-889a.a.) with a molecular weight of 100.8 kDa. It features a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ACO1 protein solution (0.5 mg/ml) is prepared in a 20 mM Tris-HCl buffer with a pH of 8.0. The buffer also contains 2 mM DTT, 100 mM NaCl, and 10% glycerol.
Purity
SDS-PAGE analysis indicates a purity greater than 90%.
Stability
For optimal storage, refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For longer-term storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Synonyms
Onitase 1 Soluble, IRP1, IREB1, IREBP, Citrate hydro-lyase, Iron regulatory protein 1, Ferritin repressor protein, Iron-responsive element-binding protein 1, ACONS, Aconitate Hydratase, EC 4.2.1.3, Aconitase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSNPFAH LAEPLDPVQP GKKFFNLNKL EDSRYGRLPF SIRVLLEAAI RNCDEFLVKK QDIENILHWN VTQHKNIEVP FKPARVILQD FTGVPAVVDF AAMRDAVKKL GGDPEKINPV CPADLVIDHS IQVDFNRRAD SLQKNQDLEF ERNRERFEFL KWGSQAFHNM RIIPPGSGII HQVNLEYLAR VVFDQDGYYY PDSLVGTDSH TTMIDGLGIL GWGVGGIEAE AVMLGQPISM VLPQVIGYRL MGKPHPLVTS TDIVLTITKH LRQVGVVGKF VEFFGPGVAQ LSIADRATIA NMCPEYGATA AFFPVDEVSI TYLVQTGRDE EKLKYIKKYL QAVGMFRDFN DPSQDPDFTQ VVELDLKTVV PCCSGPKRPQ DKVAVSDMKK DFESCLGAKQ GFKGFQVAPE HHNDHKTFIY DNTEFTLAHG SVVIAAITSC TNTSNPSVML GAGLLAKKAV DAGLNVMPYI KTSLSPGSGV VTYYLQESGV MPYLSQLGFD VVGYGCMTCI GNSGPLPEPV VEAITQGDLV AVGVLSGNRN FEGRVHPNTR ANYLASPPLV IAYAIAGTIR IDFEKEPLGV NAKGQQVFLK DIWPTRDEIQ AVERQYVIPG MFKEVYQKIE TVNESWNALA TPSDKLFFWN SKSTYIKSPP FFENLTLDLQ PPKSIVDAYV LLNLGDSVTT DHISPAGNIA RNSPAARYLT NRGLTPREFN SYGSRRGNDA VMARGTFANI RLLNRFLNKQ APQTIHLPSG EILDVFDAAE RYQQAGLPLI VLAGKEYGAG SSRDWAAKGP FLLGIKAVLA ESYERIHRSN LVGMGVIPLE YLPGENADAL GLTGQERYTI IIPENLKPQM KVQVKLDTGK TFQAVMRFDT DVELTYFLNG GILNYMIRKM AK

Product Science Overview

Structure and Function

Aconitase-1 is a cytosolic protein that contains an iron-sulfur cluster. This cluster is essential for its enzymatic activity. The protein has two distinct functions depending on the iron levels within the cell:

  1. Enzymatic Function: When cellular iron levels are sufficient, Aconitase-1 binds to a 4Fe-4S cluster and functions as an aconitase. In this role, it catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate in the TCA cycle. This reaction is crucial for cellular energy production and metabolism .

  2. Regulatory Function: When cellular iron levels are low, the 4Fe-4S cluster disassembles, and Aconitase-1 undergoes a conformational change to become an iron regulatory protein (IRP1). In this form, it binds to iron-responsive elements (IREs) in the untranslated regions (UTRs) of specific mRNAs. This binding regulates the translation of ferritin mRNA and the stability of transferrin receptor mRNA, thereby maintaining iron homeostasis .

Recombinant Aconitase-1

Recombinant Aconitase-1 is produced using recombinant DNA technology, typically expressed in Escherichia coli. The recombinant protein is purified to high levels of purity, often exceeding 90%, and is used in various research applications, including studies on iron metabolism, enzyme kinetics, and protein-protein interactions .

Applications in Research

Recombinant Aconitase-1 is widely used in biochemical and molecular biology research. Some of its applications include:

  • Studying Iron Metabolism: Researchers use recombinant Aconitase-1 to investigate the mechanisms of iron regulation in cells and the role of iron in various diseases.
  • Enzyme Kinetics: The enzyme’s activity can be studied to understand its catalytic mechanisms and the effects of different substrates and inhibitors.
  • Protein-Protein Interactions: Recombinant Aconitase-1 is used to study its interactions with other proteins involved in iron metabolism and the TCA cycle.

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