ACBD6 Human

Acyl-CoA Binding Domain Containing 6 Human Recombinant

Cat. No.

BT22471

Source

Escherichia Coli.

Synonyms

Acyl-CoA Binding Domain Containing 6, Acyl-Coenzyme A Binding Domain Containing 6, Acyl-CoA-Binding Domain-Containing Protein 6, ACBD6.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

ACBD6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (1-282aa) and having a molecular mass of 33.5kDa.

ACBD6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Acyl-CoA binding domain containing 6, also known as ACBD6, is a protein that plays a role in binding long-chain acyl-coenzyme A molecules. It exhibits a preference for unsaturated C18:1 CoA, with lower affinity for unsaturated C20:4-CoA and weak affinity for saturated C16:0-CoA. ACBD6 does not bind fatty acids. This protein is found in various tissues including the placenta, umbilical cord blood, CD34-positive hematopoietic progenitor cells, and bone marrow. ACBD6 has been linked to endotheliitis.

Description

Recombinant human ACBD6, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 305 amino acids (residues 1-282) with a 23 amino acid His-tag at the N-terminus, resulting in a molecular weight of 33.5 kDa. The protein is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, sterile-filtered solution.

Formulation

The ACBD6 protein is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.

Purity

The purity of the ACBD6 protein is greater than 90% as determined by SDS-PAGE analysis.

Synonyms

Acyl-CoA Binding Domain Containing 6, Acyl-Coenzyme A Binding Domain Containing 6, Acyl-CoA-Binding Domain-Containing Protein 6, ACBD6.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASSFLP AGAITGDSGG ELSSGDDSGE VEFPHSPEIE ETSCLAELFE KAAAHLQGLI QVASREQLLY LYARYKQVKV GNCNTPKPSF FDFEGKQKWE AWKALGDSSP SQAMQEYIAV VKKLDPGWNP QIPEKKGKEA NTGFGGPVIS SLYHEETIRE EDKNIFDYCR ENNIDHITKA IKSKNVDVNV KDEEGRALLH WACDRGHKEL VTVLLQHRAD INCQDNEGQT ALHYASACEF LDIVELLLQS GADPTLRDQD GCLPEEVTGC KTVSLVLQRH TTGKA.

Product Science Overview

Structure and Function

ACBD6 is a protein coding gene that encodes a protein with a molecular mass of approximately 32.2 kDa. The protein contains an acyl-CoA-binding domain near its N-terminus and two ankyrin repeat motifs near its C-terminus. These structural features are essential for its function in binding long-chain acyl-coenzyme A (acyl-CoA) molecules.

The primary function of ACBD6 is to bind long-chain acyl-CoA molecules with a strong preference for unsaturated C18:1-CoA, lower affinity for unsaturated C20:4-CoA, and very weak affinity for saturated C16:0-CoA . Notably, ACBD6 does not bind free fatty acids. This binding activity is crucial for the sequestration, transport, and distribution of long-chain acyl-CoAs within the cell.

Expression and Localization

ACBD6 is expressed in various human tissues, with notable expression in the placenta and spleen. It is also detected in CD34-positive progenitor cells, cord blood, bone marrow, and undifferentiated placenta-derived stromal embryo-like cells. Immunohistochemical analysis has revealed its presence in hematopoietic CD34-positive progenitors and hemangioblasts.

Biological Significance

The binding of long-chain acyl-CoAs by ACBD6 is significant for several cellular processes, including lipid metabolism and gene regulation. Long-chain acyl-CoAs are intermediates in lipid metabolism and can function as signaling molecules. By binding these molecules, ACBD6 plays a role in their cellular sequestration and transport, which is essential for maintaining cellular lipid homeostasis.

Clinical Relevance

Mutations in the ACBD6 gene have been associated with neurodevelopmental disorders with progressive movement abnormalities and combined pituitary hormone deficiency . These associations highlight the importance of ACBD6 in normal cellular function and development.

Research and Applications

Recombinant ACBD6 protein is used in research to study its binding properties and role in lipid metabolism. Studies using recombinant protein expressed in E. coli have shown that ACBD6 binds acyl-CoA substrates with varying affinities, suggesting its potential as a target for therapeutic interventions in disorders related to lipid metabolism.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

ACBD6 Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service