ABO Human
ABO Blood Group Human Recombinant
Cat. No.
BT2045
Source
Escherichia Coli.
Synonyms
Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, A transferase, Histo-blood group B transferase, B transferase, NAGAT, ABO, GTB, A3GALNT, A3GALT1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ABO Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (54-354 a.a) and having a molecular mass of 37.4kDa.
ABO is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
ABO is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
NAGAT (ABO), part of the glycosyltransferase 6 family, forms the foundation of the ABO blood group system. This system, among the earliest discovered, is determined by the specific allele an individual carries. The ABO histo-blood group consists of three carbohydrate antigens: A, B, and H. Individuals with A, B, or AB blood types possess glycosyltransferase activity that modifies the H antigen into either the A antigen (by adding UDP-GalNAc) or the B antigen (by adding UDP-Gal). Conversely, individuals with type O blood lack this enzyme activity.
Description
Recombinant Human ABO, synthesized in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 322 amino acids (54-354 a.a), resulting in a molecular weight of 37.4kDa. The ABO protein is engineered with a 21 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The ABO protein is supplied in a solution at a concentration of 0.5mg/ml. The solution is buffered with 20mM Tris-HCl at pH 8.0 and also contains 2mM DTT, 20% glycerol, and 200mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the ABO protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, A transferase, Histo-blood group B transferase, B transferase, NAGAT, ABO, GTB, A3GALNT, A3GALT1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP.
Product Science Overview
Discovery and Significance
Karl Landsteiner’s groundbreaking work revealed that blood from different individuals could cause agglutination (clumping) when mixed, leading to the identification of the ABO blood groups . This discovery was crucial for the safe practice of blood transfusion, as it helped to prevent adverse reactions caused by incompatible blood types .
Antigens and Antibodies
The ABO blood group system is determined by the presence of specific antigens on the surface of red blood cells and corresponding antibodies in the plasma .
- Type A: Has A antigens on the red cells and anti-B antibodies in the plasma.
- Type B: Has B antigens on the red cells and anti-A antibodies in the plasma.
- Type AB: Has both A and B antigens on the red cells and no anti-A or anti-B antibodies in the plasma.
- Type O: Has no A or B antigens on the red cells but has both anti-A and anti-B antibodies in the plasma .
Inheritance and Genetics
Human Recombinant ABO Blood Group
The development of recombinant technology has allowed for the production of human recombinant ABO blood group antigens. This involves the use of genetic engineering to produce the antigens in a controlled laboratory environment . These recombinant antigens can be used for various applications, including:
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