ABI3 Human

ABI Family, Member 3 Human Recombinant
Cat. No.
BT22334
Source
E.coli.
Synonyms
ABI Family Member 3, ABI Gene Family Member 3, New Molecule Including SH3, SSH3BP3, NESH.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ABI3 Human Recombinant produced in E. coli is a single polypeptide chain containing 389 amino acids (1-366) and having a molecular mass of 41.4 kDa.
ABI3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ABI family member 3 (ABI3) belongs to the ABI family, which encodes proteins containing a homeobox homology domain, a proline-rich region, and an Src-homology 3 (SH3) domain. These proteins are thought to suppress cell growth and transformation, including v-Abl-mediated transformation, and regulate cell motility by influencing actin polymerization in lamellipodia and filopodia. ABI3 interacts with p21-activated kinase to hinder the spread of tumor cells and inhibit cell migration.
Description
Recombinant human ABI3, produced in E. coli, is a single polypeptide chain consisting of 389 amino acids (residues 1-366) with a molecular weight of 41.4 kDa. A 23-amino acid His-tag is fused to the N-terminus of ABI3. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A sterile, filtered solution that is colorless.
Formulation
The ABI3 solution is supplied in a 20mM Tris-HCl buffer with a pH of 8.0, containing 0.4M Urea and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is advisable to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be higher than 85% based on SDS-PAGE analysis.
Synonyms
ABI Family Member 3, ABI Gene Family Member 3, New Molecule Including SH3, SSH3BP3, NESH.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAELQQL QEFEIPTGRE ALRGNHSALL RVADYCEDNY VQATDKRKAL EETMAFTTQA LASVAYQVGN LAGHTLRMLD LQGAALRQVE ARVSTLGQMV NMHMEKVARR EIGTLATVQR LPPGQKVIAP ENLPPLTPYC RRPLNFGCLD DIGHGIKDLS TQLSRTGTLS RKSIKAPATP ASATLGRPPR IPEPVHLPVV PDGRLSAASS ASSLASAGSA EGVGGAPTPK GQAAPPAPPL PSSLDPPPPP AAVEVFQRPP TLEELSPPPP DEELPLPLDL PPPPPLDGDE LGLPPPPPGF GPDEPSWVPA SYLEKVVTLY PYTSQKDNEL SFSEGTVICV TRRYSDGWCE GVSSEGTGFF PGNYVEPSC

Product Science Overview

Classification and Structure

ABI3 belongs to the ABI family, which includes several other members that share similar structural features. The human recombinant ABI3 protein is produced in E. coli and consists of a single polypeptide chain containing 389 amino acids (1-366) with a molecular mass of approximately 41.4 kDa . It is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques .

Biological Properties and Functions

ABI3 plays a significant role in negatively regulating cell growth and transformation. It is involved in cellular transformation through v-Abl and mediates cell motility by regulating actin polymerization . Additionally, ABI3 impedes ectopic metastasis of tumor cells and inhibits cell migration through its interaction with p21-activated kinase .

Expression Patterns and Tissue Distribution

The expression patterns and tissue distribution of ABI3 are not extensively detailed in the available literature. However, as a member of the ABI family, it is likely to be expressed in various tissues where actin polymerization and cell motility are critical.

Modes of Action and Regulatory Mechanisms

ABI3 functions as part of the Abi/WAVE complex, which is essential for actin polymerization . This complex regulates the formation of actin filaments, which are necessary for various cellular processes, including cell movement and shape maintenance . The interaction of ABI3 with other proteins, such as p21-activated kinase, highlights its role in signaling pathways that control cell migration and metastasis .

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