ABHD14B Human
Abhydrolase Domain Containing 14B Human Recombinant
Cat. No.
BT26461
Source
E.coli.
Synonyms
Abhydrolase domain containing protein 14B, CIB, CCG1-interacting factor B, cell cycle gene 1-interacting factor B, EC 3.1.11.6, EC 3.1.21.4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ABHD14B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (1-210) and having a molecular mass of 25.0kDa.
ABHD14B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
ABHD14B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
ABHD14B, a member of the AB hydrolase superfamily, possesses an alpha/beta hydrolase fold, a catalytic domain prevalent in various hydrolytic enzymes. This fold is widely observed in hydrolytic enzymes from diverse phylogenetic origins and catalytic functions. The Ab hydrolase domain containing gene subfamily comprises 15 members, mostly uncharacterized. ABHD14B exhibits hydrolase activity with p-nitrophenyl butyrate (in vitro) and possesses transcriptional activation capabilities.
Description
Recombinant human ABHD14B, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 234 amino acids (residues 1-210) with a molecular weight of 25.0 kDa. It includes an N-terminal 24-amino acid His-tag and is purified using proprietary chromatographic methods.
Physical Appearance
Sterile, colorless solution, free from particulate matter.
Formulation
The ABHD14B solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
Abhydrolase domain containing protein 14B, CIB, CCG1-interacting factor B, cell cycle gene 1-interacting factor B, EC 3.1.11.6, EC 3.1.21.4.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSE TWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMY SLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGA GHPCYLDKPE EWHTGLLDFL QGLQ
Product Science Overview
Gene and Protein Information
- Gene Symbol: ABHD14B
- Aliases: CCG1/TAFII250-Interacting Factor B (CIB), Epididymis Secretory Sperm Binding Protein, Protein ABHD14B, among others .
- Location: The ABHD14B gene is located on chromosome 3 .
- Protein Structure: The ABHD14B protein contains an alpha/beta hydrolase fold, which is common to many hydrolytic enzymes .
Biological Functions
ABHD14B has been implicated in several biological processes:
- Hydrolase Activity: It exhibits hydrolase activity towards various substrates, including p-nitrophenyl butyrate, p-nitrophenyl acetate, and p-nitrophenyl octanoate, with a strong preference for p-nitrophenyl acetate .
- Deacetylase Activity: In vitro studies suggest that ABHD14B acts as an atypical protein-lysine deacetylase, catalyzing the deacetylation of lysine residues using CoA as a substrate .
- Transcription Regulation: ABHD14B may play a role in activating transcription .
Expression and Localization
Recombinant ABHD14B
Recombinant human ABHD14B protein is often produced with an N-terminal His-tag for ease of purification. It is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant protein retains the hydrolase activity observed in the native protein and is used in various biochemical assays to study its function and regulation .
Research and Clinical Implications
The study of ABHD14B is ongoing, with research focusing on its enzymatic activities and potential roles in cellular processes. Understanding the function and regulation of ABHD14B could provide insights into its involvement in diseases and its potential as a therapeutic target.
For more detailed information, you can refer to resources like GeneCards and Bio-Techne.
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