AASDHPPT Human
Aminoadipate-Semialdehyde Dehydrogenase-Phosphopantetheinyl Transferase Human Recombinant
Cat. No.
BT2013
Source
Escherichia Coli.
Synonyms
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, 4'-phosphopantetheinyl transferase, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, LYS5 ortholog, AASDHPPT, LYS2, LYS5, CGI-80, DKFZp566E2346.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
AASDHPPT Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 316 amino acids (14-309 a.a.) and having a molecular mass of 36.4kDa. The AASDHPPT is purified by proprietary chromatographic techniques.
Product Specs
Introduction
AASDHPPT, a member of the P-Pant transferase superfamily, catalyzes the post-translational modification of proteins by phosphopantetheine. It facilitates the transfer of the 4'-phosphopantetheine group from coenzyme A to a serine residue on various acceptor proteins, including the acyl carrier domain of FASN (in vitro). Functionally similar to Saccharomyces cerevisiae LYS5, which is crucial for activating alpha-aminoadipate dehydrogenase in the lysine biosynthesis pathway, AASDHPPT is expressed in various tissues like the heart, skeletal muscle, placenta, testis, brain, pancreas, liver, and kidney. Defects in the human AASDHPPT gene have been implicated in pipecolic acidemia.
Description
Recombinant human AASDHPPT, fused with a 21 amino acid His tag at its N-terminus, is produced in E. coli. This purified protein is a single, non-glycosylated polypeptide chain comprising 316 amino acids (14-309 a.a.) with a molecular weight of 36.4kDa. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The AASDHPPT solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% Glycerol.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of AASDHPPT is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, 4'-phosphopantetheinyl transferase, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, LYS5 ortholog, AASDHPPT, LYS2, LYS5, CGI-80, DKFZp566E2346.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEGVRWAFSC GTWLPSRAEW LLAVRSIQPE EKERIGQFVF ARDAKAAMAG RLMIRKLVAE KLNIPWNHIR LQRTAKGKPV LAKDSSNPYP NFNFNISHQG DYAVLAAEPE LQVGIDIMKT SFPGRGSIPE FFHIMKRKFT NKEWETIRSF KDEWTQLDMF YRNWALKESF IKAIGVGLGF ELQRLEFDLS PLNLDIGQVY KETRLFLDGE EEKEWAFEES KIDEHHFVAV ALRKPDGSRH QDVPSQDDSK PTQRQFTILN FNDLMSSAVP MTPEDPSFWD CFCFTEEIPI RNGTKS.
Product Science Overview
Function and Mechanism
AASDHPPT is involved in the post-translational modification of proteins by transferring the 4’-phosphopantetheine moiety from coenzyme A to a serine residue of target proteins. This modification is essential for the activation of various enzymes, including the alpha-aminoadipate dehydrogenase, which converts alpha-aminoadipate semialdehyde to alpha-aminoadipate in the lysine biosynthetic pathway .
Biological Significance
Pathways and Interactions
Research and Clinical Implications
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