AAMDC Human

Adipogenesis Associated, Mth938 Domain Containing Human Recombinant
Cat. No.
BT22196
Source
Escherichia Coli.
Synonyms
Adipogenesis Associated, Mth938 Domain Containing, Adipogenesis Associated Mth938 Domain-Containing Protein , C11orf67, Chromosome 11 Open Reading Frame 67, Mth938 Domain-Containing Protein, UPF0366 Protein C11orf67, PTD015, CK067,AAMDC.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

AAMDC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 145 amino acids (1-122 a.a) and having a molecular mass of 15.7 kDa.
AAMDC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Adipogenesis Associated, Mth938 Domain Containing, also known as AAMDC, is a member of the AAMDC family. It is involved in preadipocyte differentiation and adipogenesis.
Description
Recombinant human AAMDC protein was expressed in E. coli. It is a single, non-glycosylated polypeptide chain that contains 145 amino acids (1-122 a.a) and has a molecular mass of 15.7 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile filtered.
Formulation
AAMDC protein solution (1 mg/ml) is supplied in phosphate buffered saline (pH 7.4) containing 20% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), store the solution at 4°C. For long-term storage, store the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE.
Synonyms
Adipogenesis Associated, Mth938 Domain Containing, Adipogenesis Associated Mth938 Domain-Containing Protein , C11orf67, Chromosome 11 Open Reading Frame 67, Mth938 Domain-Containing Protein, UPF0366 Protein C11orf67, PTD015, CK067,AAMDC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTSPEIA SLSWGQMKVK GSNTTYKDCK VWPGGSRTWD WRETGTEHSP GVQPADVKEV VEKGVQTLVI GRGMSEALKV PSSTVEYLKK HGIDVRVLQT EQAVKEYNAL VAQGVRVGGV FHSTC.

Product Science Overview

Gene and Protein Information
  • Gene Symbol: AAMDC
  • Chromosomal Location: 11q14.1
  • Protein: Adipogenesis Associated Mth938 Domain Containing Protein
  • UniProtKB/Swiss-Prot ID: Q9H7C9

The AAMDC gene encodes a protein that contains the Methanobacterium thermoautotrophicum-938 (Mth938) domain, which is highly conserved across various species, including humans, mice, rats, cows, chickens, and zebrafish . This domain is crucial for the protein’s function in adipogenesis.

Function and Expression

AAMDC is highly expressed in adipose tissue and is involved in the positive regulation of fat cell differentiation . It acts upstream of or within the negative regulation of the apoptotic process and the positive regulation of transcription by RNA polymerase II . The protein is predicted to be active in the cytoplasm.

Studies have shown that overexpression of AAMDC promotes the activity of transcription factors such as Elk1 and Jun, likely through the MAPK pathway . Additionally, it enhances the transcription activity of CREB1, a protein necessary for adipogenic conversion of preadipocytes . Overexpression of AAMDC reduces the proliferation of preadipocytes and promotes their differentiation into adipocytes, while knockdown of AAMDC increases apoptosis during adipogenesis .

Clinical Relevance

Diseases associated with AAMDC include Agammaglobulinemia 6, a condition characterized by a lack of gamma globulins in the blood, leading to a weakened immune system . The gene’s role in adipogenesis also makes it a potential target for research in obesity and metabolic disorders.

Research and Applications

The recombinant form of the AAMDC protein is used in various research applications to study its function and role in adipogenesis. Researchers utilize techniques such as RT-PCR, Western blot analysis, and confocal microscopy to investigate the expression and localization of AAMDC in different tissues .

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