AAGAB Human

Alpha & Gamma-Adaptin Binding Protein Human Recombinant
Cat. No.
BT22132
Source
Escherichia Coli.
Synonyms
P34, Alpha- and gamma-adaptin-binding protein p34.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs
In Stock
Quick Inquiry

Description

AAGAB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (1-315 a.a.) and having a molecular mass of 36.7kDa.
AAGAB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The Alpha & Gamma-Adaptin Binding Protein (AAGAB), also called P34, plays a role in membrane traffic. It interacts with AP1G1 and AP2A1. AAGAB is found in high concentrations in skin and keratinocytes, with the highest expression levels in the adrenal gland, rectum, and thymus.
Description
Recombinant human AAGAB, produced in E. coli, is a single, non-glycosylated polypeptide chain comprised of 335 amino acids (specifically, amino acids 1 through 315). It has a molecular mass of 36.7 kDa. A 20 amino acid His-tag is fused to the N-terminus of AAGAB, which is then purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, filtered, and clear solution.
Formulation
The AAGAB protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For longer storage, it should be frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
P34, Alpha- and gamma-adaptin-binding protein p34.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAGVPCALV TSCSSVFSGD QLVQHILGTE DLIVEVTSND AVRFYPWTID NKYYSADINL CVVPNKFLVT AEIAESVQAF VVYFDSTQKS GLDSVSSWLP LAKAWLPEVM ILVCDRVSED GINRQKAQEW CIKHGFELVE LSPEELPEED DDFPESTGVK RIVQALNANV WSNVVMKNDR NQGFSLLNSL TGTNHSIGSA DPCHPEQPHL PAADSTESLS DHRGGASNTT DAQVDSIVDP MLDLDIQELA SLTTGGGDVE NFERLFSKLK EMKDKAATLP HEQRKVHAEK VAKAFWMAIG GDRDEIEGLS SDEEH.

Product Science Overview

Introduction

Alpha and Gamma-Adaptin Binding Protein (AAGAB), also known as p34, is a protein encoded by the AAGAB gene. This protein interacts with the alpha- and gamma-adaptin subunits of clathrin adaptor protein complexes, which are crucial for clathrin-mediated vesicle trafficking . Clathrin-mediated endocytosis is a fundamental cellular process that facilitates the internalization of various molecules, including nutrients, hormones, and other signaling molecules .

Gene and Protein Structure

The AAGAB gene is located on chromosome 15 and encodes a protein that is approximately 34 kDa in size . The protein is predominantly cytosolic and is involved in the regulation of clathrin adaptor protein complexes AP1 and AP2 . These complexes play a pivotal role in the formation of clathrin-coated vesicles, which are essential for intracellular trafficking between the plasma membrane, trans-Golgi network, and endosomes .

Function and Mechanism

AAGAB functions as an assembly chaperone for the AP1 and AP2 clathrin adaptor complexes . It binds to and stabilizes the gamma and alpha subunits of these complexes, promoting their assembly and function. This stabilization is crucial for the proper sorting and trafficking of cargo proteins within the cell . Mutations in the AAGAB gene can disrupt this process, leading to various cellular dysfunctions and diseases .

Clinical Significance

Mutations in the AAGAB gene are associated with type I punctate palmoplantar keratoderma, a skin disorder characterized by the development of hyperkeratotic lesions on the palms and soles . Additionally, AAGAB has been identified as a potential biomarker for certain cancers, including breast cancer . Its expression levels can influence the response to neoadjuvant chemotherapy, making it a valuable target for diagnostic and prognostic applications .

Research and Applications

Recent studies have highlighted the importance of AAGAB in maintaining cellular homeostasis and its potential role in disease pathogenesis . Understanding the molecular mechanisms underlying AAGAB function can provide insights into the development of therapeutic strategies for diseases associated with clathrin-mediated trafficking defects .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.